How to Use the ISO 21702 Method to Find Out the Virus in Plastic and Another Non-Porous Surface?
With the massive development in science, there are several procedures to find out the viruses. But, here the ISO 21702 is the proper method that delivers the best result. We have commonly done this surface test with various viruses, and it can be substituted based on the customer's wants. It is the standard method to test the overall ability of plastics and other common materials to kill the different viruses active on the material's surface. We did this method for 24 hours of content contact with the virus, and it conducts via with the help of the Influenza a virus and Feline calicivirus.
This method is commonly suggested to test the antiviral surface test method for evaluating the overall antimicrobial activity of treated plastic and non-porous items. Antimicrobial plastic ensures to meet all wants of this method ISO 21702 at the time of conditioned is supported the environment treated plastics that fails to work as intended. It is necessary to incorporate environmental conditions into the different processes of the testing, such as humidity, UV exposure which affect the antiviral plastic. Most customers wish to go with the durability testing process with this ISO 2170 testing to find out the different viruses' overall antimicrobial performance in this product. Here you can use both methods via situ Bioscience product test laboratory, which assists in product development and supports performance when the testing time is reduced.
How will you test when the material is antiviral?
The most important route of the virus spread is the surface and other material. When you are under the COVID -19 pandemic, the people have to be accustomed to often wiping down any touched surface. At the same time, there is an excellent demand of upcoming generation for growth of antiviral surface which faster inactivates to any contaminating virus particles. The material developer has two methods such naturally antiviral materials and incorporating antiviral additives into the products.
This method is specially designed to test plastic and other non-porous surface. This process occurs with a predetermined concentration of virus over the test surface and reference surface, which are left humidified at room temperatures for 24 hours. Apart from that, this type of test result is reliable and meaningful.
Let us explain different controls and how to test surfaces from below:
If you are testing the given surface for an antiviral activity product, at first, we use the 5x5 cm squares and are also committed to testing in the triplicate at all times. You have taken a small volume of virus up to 200 µl, which must be filled up to one-fifth of a milliliter, and it must add in a piece of the test surface and the control surface. Once it is agreed upon, the contact time must be between the test surface and virus for 24 hours, and they have to recover this surviving virus in the part of the liquid media.
Next, we have to find out how the respective virus survived over this test surface and a control surface. This process does not occur directly, but it can observe the overall damages caused by the infection to the mammalian host cell. We have loaded the surviving virus here into the cell cultured in the part of the 96 plates. When the virus is left with cultured cells for sufficient time, it allows over infection to progress. At last, the cells become fixed and stained in the form of violet and stain to discriminate with wells containing cells infected with a different virus. Then it shows the score as either infected or not infected, but this score helps determine the R-value for the overall tested surface.
Method to calculate the antiviral activity:
Using the ISO 21702 test method is the right option to find out the virus over the surface of interest relative to having a proper control sample. The overall reduction is also called the R-Value. This R-value differs from the overall amount of virus recovered from the given reference and tests the sample. To claim the overall antiviral activity, the sample needs to meet the R-value is more than 1 if the R-value is equivalent to a reduction of the 90% of infectious in the sample to control samples. If the R-value is more than 2, which has a 99% reduction, and if the R-value is 3, which has 99.9% reduction, it gives the customer more comfortable producing the overall product at all times. The R-value must become a relative measurement because the viruses tend to degrade and become inactive over this surface at this time. Hence the R-value explains to us that the additional virus inactivation is due to the sample of interest. On reporting this over values of the R, it controls for variation and tests when it is the contribution of treatment to the inactivation of the virus.
With the help of the ISO 21702, ensure on the cultured host cells to find out the overall infection virus. When you find out the overall amount of the infection virus, get back from this test surface by adding the recovered virus over the host cells. Hence, this type of virus killed this type of host cells in the characteristic method, so the infection virus recovered and the host cell dies.
We have used this type of testwhen the test material interfaces with different hot cells. If the test material is out, something into the part of the culture media creates cells resistant to the virus. In this process, they add the liquid media to the test and reference surface and wait up to 5 minutes. Again recover the media and add some virus to the recovered media and wait up to 30 minutes. Then you need to add the same amount to the media which has not been in touch with either the test or other reference material. When you have many more hoots cells than measured in the option media, only a sample and help to test become invalidated.
VRS controls must make sure the result of the antiviral test is strictly interpreted if the condition is valid, and it must be checked out and give the best output at all times. Here the ISO 21702 will control the VRS method, and if it is a single positive and single negative control, then it is treated as test and also reference materials. The validated material shows the upbeat control with the help of the confirms, which is inert and marginally. It becomes necessary when you come to reference material when it itself is antiviral. Hence the positive control makes sure that the assay that we are performing correctly and conditions to reveal the veridical property will be met. Finally, it allows us to compare the overall performance of the assay and across the various operations.
If there is a well-performed test, then a massive number of controls developed the number of the test condition.. Here the control becomes hard to know and interpret the test, and you must know-how will go wrong. Hence this method is highly welcomed by everyone to test the virus in a simple and effective process.
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